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ssaav vector constructs expressing cas9  (Addgene inc)


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    Structured Review

    Addgene inc ssaav vector constructs expressing cas9
    Ssaav Vector Constructs Expressing Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 369 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ssaav+vector+constructs+expressing+cas9/SP-Cas9+(Plasmid+%2362731)/pmc06360332__mmc2-310-30-56
    Average 96 stars, based on 369 article reviews
    ssaav vector constructs expressing cas9 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Bioprocessing:

    Article Title: Novel Chimeric Gene Therapy Vectors Based on Adeno-Associated Virus and Four Different Mammalian Bocaviruses
    Article Snippet: .. In this backbone, two AAV ITRs, one of which carries a mutation of the terminal resolution site, flank the expression cassettes and allow for their packaging as scAAV genomes.88 Oversized ssAAV vector constructs expressing Cas9 from Streptococcus pyogenes (Sp) were derived from our previously reported Cas9 expression plasmid3 containing the SpCas9 cDNA from the Church lab (Addgene plasmid 41815). .. This variant was now replaced with the SpCas9 cDNA from the Zhang lab, by PCR-amplifying this cDNA from former Addgene plasmid 49535 (in the meantime replaced by Addgene by plasmid 52961) using primers 46 and 47 and by cloning the NheI/ClaI-digested PCR product into our appropriately cleaved original AAV/SpCas9 plasmid,3 resulting in pAAVFZSpCas9.

    Mutagenesis:

    Article Title: Novel Chimeric Gene Therapy Vectors Based on Adeno-Associated Virus and Four Different Mammalian Bocaviruses
    Article Snippet: .. In this backbone, two AAV ITRs, one of which carries a mutation of the terminal resolution site, flank the expression cassettes and allow for their packaging as scAAV genomes.88 Oversized ssAAV vector constructs expressing Cas9 from Streptococcus pyogenes (Sp) were derived from our previously reported Cas9 expression plasmid3 containing the SpCas9 cDNA from the Church lab (Addgene plasmid 41815). .. This variant was now replaced with the SpCas9 cDNA from the Zhang lab, by PCR-amplifying this cDNA from former Addgene plasmid 49535 (in the meantime replaced by Addgene by plasmid 52961) using primers 46 and 47 and by cloning the NheI/ClaI-digested PCR product into our appropriately cleaved original AAV/SpCas9 plasmid,3 resulting in pAAVFZSpCas9.

    Expressing:

    Article Title: Novel Chimeric Gene Therapy Vectors Based on Adeno-Associated Virus and Four Different Mammalian Bocaviruses
    Article Snippet: .. In this backbone, two AAV ITRs, one of which carries a mutation of the terminal resolution site, flank the expression cassettes and allow for their packaging as scAAV genomes.88 Oversized ssAAV vector constructs expressing Cas9 from Streptococcus pyogenes (Sp) were derived from our previously reported Cas9 expression plasmid3 containing the SpCas9 cDNA from the Church lab (Addgene plasmid 41815). .. This variant was now replaced with the SpCas9 cDNA from the Zhang lab, by PCR-amplifying this cDNA from former Addgene plasmid 49535 (in the meantime replaced by Addgene by plasmid 52961) using primers 46 and 47 and by cloning the NheI/ClaI-digested PCR product into our appropriately cleaved original AAV/SpCas9 plasmid,3 resulting in pAAVFZSpCas9.

    Plasmid Preparation:

    Article Title: Novel Chimeric Gene Therapy Vectors Based on Adeno-Associated Virus and Four Different Mammalian Bocaviruses
    Article Snippet: .. In this backbone, two AAV ITRs, one of which carries a mutation of the terminal resolution site, flank the expression cassettes and allow for their packaging as scAAV genomes.88 Oversized ssAAV vector constructs expressing Cas9 from Streptococcus pyogenes (Sp) were derived from our previously reported Cas9 expression plasmid3 containing the SpCas9 cDNA from the Church lab (Addgene plasmid 41815). .. This variant was now replaced with the SpCas9 cDNA from the Zhang lab, by PCR-amplifying this cDNA from former Addgene plasmid 49535 (in the meantime replaced by Addgene by plasmid 52961) using primers 46 and 47 and by cloning the NheI/ClaI-digested PCR product into our appropriately cleaved original AAV/SpCas9 plasmid,3 resulting in pAAVFZSpCas9.

    Construct:

    Article Title: Novel Chimeric Gene Therapy Vectors Based on Adeno-Associated Virus and Four Different Mammalian Bocaviruses
    Article Snippet: .. In this backbone, two AAV ITRs, one of which carries a mutation of the terminal resolution site, flank the expression cassettes and allow for their packaging as scAAV genomes.88 Oversized ssAAV vector constructs expressing Cas9 from Streptococcus pyogenes (Sp) were derived from our previously reported Cas9 expression plasmid3 containing the SpCas9 cDNA from the Church lab (Addgene plasmid 41815). .. This variant was now replaced with the SpCas9 cDNA from the Zhang lab, by PCR-amplifying this cDNA from former Addgene plasmid 49535 (in the meantime replaced by Addgene by plasmid 52961) using primers 46 and 47 and by cloning the NheI/ClaI-digested PCR product into our appropriately cleaved original AAV/SpCas9 plasmid,3 resulting in pAAVFZSpCas9.

    Derivative Assay:

    Article Title: Novel Chimeric Gene Therapy Vectors Based on Adeno-Associated Virus and Four Different Mammalian Bocaviruses
    Article Snippet: .. In this backbone, two AAV ITRs, one of which carries a mutation of the terminal resolution site, flank the expression cassettes and allow for their packaging as scAAV genomes.88 Oversized ssAAV vector constructs expressing Cas9 from Streptococcus pyogenes (Sp) were derived from our previously reported Cas9 expression plasmid3 containing the SpCas9 cDNA from the Church lab (Addgene plasmid 41815). .. This variant was now replaced with the SpCas9 cDNA from the Zhang lab, by PCR-amplifying this cDNA from former Addgene plasmid 49535 (in the meantime replaced by Addgene by plasmid 52961) using primers 46 and 47 and by cloning the NheI/ClaI-digested PCR product into our appropriately cleaved original AAV/SpCas9 plasmid,3 resulting in pAAVFZSpCas9.



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    ssaav vector constructs expressing cas9  (Addgene inc)
    96
    Addgene inc ssaav vector constructs expressing cas9
    Ssaav Vector Constructs Expressing Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ssaav+vector+constructs+expressing+cas9/SP-Cas9+(Plasmid+%2362731)/pmc06360332__mmc2-310-30-56
    Average 96 stars, based on 1 article reviews
    ssaav vector constructs expressing cas9 - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

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